Biologia plantarum 6:104-107, 1964 | DOI: 10.1007/BF02926664
The accentuators, pH and the principles of the effect in histological staining
- 1 Institute of Biology of Charles University, Medical Faculty, Hradec Králové, Czechoslovakia
A study was made of the influence of accentuators (phenol, aniline) and pH on the stainability of vegetable tissue (roots ofAllium cepa L.) with light green and methylene blue with reference to the theory of the electrostatic basis of staining. - The results obtained on the whole confirmPischinger's theory of the electrostatic basis of staining for light green only, but with methylene blue the situation was found to be much more complicated. Only the alkalization of a watery solution of methylene blue increases its staining ability, whereas the accentuators markedly disturb staining. Prischinge's theory is thus not of general validity, but applies only to one factor of this complicated process. Electrostatic influences acting on staining ability are not so important as other factors in this complicated process in some dyes.
Received: March 9, 1963; Published: June 1, 1964 Show citation
References
- DavenpoRt, H. A.: Histological and Histochemical Technics. - Philadelphia 1960.
- HeRtwig, G.: Die mikroskopisch-anatomischen Untersuchungsmethoden. Ein Handbuch der mikroskopischen Anatomie des Mensehen. Teil I., S. 69-105, 1929.
- Pappenhaim, a.: Farbchemie 1901.
- PischingeR, A.: Die Lage des isoelektrischen Punktes histologischer Elements als Ursache ihrer verschiedenen Färbbarkeit.- Ztschr. f. Zellforsch. u. Mikr. Anat. 3: 169-197, 1926.
Go to original source... - Singer, M.: Factors which Control the Staining of Tissue. Sections with Acid and Basic Dyes. - In: International Review of Cytology. Vol. I., pp. 211-255. - New York 1952. Go to original source...