biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 9:102-108, 1967 | DOI: 10.1007/BF02929726

Share of carbohydrates in the humus substances composition

Jakoslava HavrÁnkovÁ1
1 Department of Plant Physiology and Soil Biology, Charles University, Praha

The effect of different concentrations of hydrochloric acid on the qualitative an quantitative content of carbohydrates in humus fraction hydrolyzates was investigated. Three humic acid preparations were subjected to the usual procedure for obtaining fractions of humic and hymatomelanic acids. The carbohydrate content of the hydrolyzates was determined after their Chromatographic separation on Whatman 1, using the Partridge system and detection with acid anilin phtalate. The results showed that the qualitative carbohydrate content in the hydrolyzates of most humus fractions of samples used was identical. With increasing acid concentrations the amount of hydrolyzable carbohydrates usually increased, the total amount of carbohydrate in the hydrolyzate not exceeding 4% . The difference in the carbohydrate content as between hydrolysis with 0-9N and 5-3N HC1 was sometimes fourfold. A higher content of hexoses than of pentoses was generally found.

Received: April 6, 1966; Published: March 1, 1967  Show citation

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HavrÁnkovÁ, J. (1967). Share of carbohydrates in the humus substances composition. Biologia plantarum, 9(2), 102-108. doi: 10.1007/BF02929726
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