biologia plantarum

Biologia plantarum 9:454-461, 1967 | DOI: 10.1007/BF02926987

Cytoplasmic Components of the Broad Bean Root Tip Cells

A. F. M. Maniruzzaman¹, K. Beneš², J. Ludvík³

¹ Czechoslovak Academy of Science, Institute of Microbiology and Institute of Experimental Botany, Praha

² Czechoslovak Academy of Science, Institute of Experimental Botany, Praha 6 - Vokovice, Czechoslovakia

³ Czechoslovak Academy of Science, Institute of Microbiology, Praha 4 - Krč, Czechoslovakia

The cytoplasmic components of broad bean (Vicia faba L.) root tip cells were studied light- and electronmicroscopically. Using a light microscope, differences were revealed between cells from the cortex and from the central cylinder. For electron microscope studies the cells near the boundary between the mentioned parts of the root tip were selected at a distance of about 2 mm from the initials. The orientation of objects during embedding made possible fairly accurate localization. No peculiar, strikingly osmiophillic bodies were seen, which could be identified without doubt as osmiophillic platelets. It follows that some of the current cytoplasmic components, perhaps more or less altered were described as osmiophillic platelets. After fixation with KMnO₄, in which case the electronmicrographs are most instructive, the leucoplasts show several inclusions, mostly only partially limited against the matrix; it is not clear whether the true membrane is concerned here. The origin of intravacuolar membraneous formations is discussed.

Received: February 27, 1967; Published: November 1, 1967  Show citation