biologia plantarum

Biologia plantarum 10:58, 1968 | DOI: 10.1007/BF02920745

On the catabolic pathways of sugars in green algae

J. Dvořáková-Hladká¹

¹ Collection of Cultures of Autotrophic Organisms, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

Respiratory metabolism of the cultures of algaeChlorella pyrenoidosa (82) andScenedesmus obliquus (125) was investigated. One part of algae were cultivated on a mineral nutrient solution, another two parts on the solution with glucose and on the solution with glucose and yeast decoction. Individual steps of respiratory metabolism-endogenous as well as in the presence of exogenous sugars-were estimated according to the response to the following inhibitors: monoiodacetate, NaF, NaN₃, and 2,4-DNP. In the last two cases, fructose, glucose-6-phosphate and fructose-1,6-diphosphate were applied in parallel for comparison. Na-monoiodacetate was found to inhibit the respiration of both endogenous and exogenous (with glucose) substrates, NaF (concentrations up to 2.5.10^-2 m) stimulated the O₂ uptake. The effect of sodium azide and 2,4-DNP depended in both strains on previous cultivation. On the basis of the results obtained, the presence of particular respiration pathways in the dissimilation of glucose is discussed. The following catabolic processes are to be considered: a) direct oxidation (with both autotrophically cultivated strains and with theChlorella cultivated on glucosecontaining medium), b) the process similar to glycolysis, which, however, does not necessarily involve the enolase (it is not inhibited by NaF) c) pentosephosphate cycle (Chlorella), and d) glycolysis, in which both algae can operate when sugars previously phosphorylated are applied.

Received: May 18, 1967; Published: January 1, 1968  Show citation