Biologia plantarum 10:275, 1968 | DOI: 10.1007/BF02921010
Gasometric method of water deficit measurement in leaves
- 1 Department of Plant Physiology, The University of Warsaw, Poland
A gasometric method was developed for measuring water deficit in leaves. For a leaf at full turgor the amount of water penetrating into the tissue after removing the air from intercellular spaces by means of a vacuum pump, is equal in volume to the gas removed from the intercellular spaces. In a leaf with a water deficit the amount of the infiltrating water is greater than the removed gas volume by the amount egual to the water deficit. Determination of the volumes of the gas removed and penetrating water enables water deficit, if any, to be calculated. Comparative measurements carried out on five plant species confirmed the correctness of the method suggested. Reduction of the measuring time allowed to eliminate completely the sources of errors associated with the growth of tissue and loss of dry weight during respiration.
Received: December 4, 1967; Published: July 1, 1968 Show citation
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