biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 11:324-326, 1969 | DOI: 10.1007/BF02921150

Purification and conservation of infective sugar beet mosaic virus

J. Chod1, J. Polák1
1 Institute of Plant Protection, Praha

Sugar beet mosaic virus (SBMV) was precipitated by polyethyleneglycol (PEG) 6000 from the cell sap of infected sugar beet leaves. After centrifugation and addition of dextrane T 10 the virus was lyophilized. Its infectious activity was demonstrated by mechanical transmission toChenopodium quinoa Willd. and to sugar beet. Stability of infectious activity of the lyophilized virus was verified.

Received: January 4, 1969; Published: July 1, 1969  Show citation

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Chod, J., & Polák, J. (1969). Purification and conservation of infective sugar beet mosaic virus. Biologia plantarum, 11(4), 324-326. doi: 10.1007/BF02921150
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    References

    1. Herbert, T. T.: Precipitation of plant virus by polyethyleneglycol.-Phytophatology53: 362, 1963.
    2. Jermoljev, E., Albrechtová, L.: Stabilization of purified potato virus X by dextrane T-10 during lyophilization.-Biol. Plant. in press 1969. Go to original source...
    3. Lindner, R. C., Kirkpatrick, H. C., Weeks, T. E.: Some factors affecting the purification of a stone fruit virus.-Phytopathology45: 574-576, 1955.
    4. Mundry, K. W., Rohmer, I. Über die mechanische Übertragung des Vergilbungsvirus der Rüben. -Phytopath. Z.31: 305-318, 1958.

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