biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 12:327-331, 1970 | DOI: 10.1007/BF02922186

Electron microscopic autoradiographic study of RNA isolated from apple-tree callus tissue labelled with 6-Benzylaminopurine-8-C14

Alena Volfová1, A. Friedrich1, L. Chvojka1
1 Department of Radiobiolology, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6-Vokovice, Czechoslovakia

In the present paper RNA from apple-tree callus tissue labelled with 6-benzyl-minopurine14) was studied. The RNA isolated from this tissue was prepared as sample for electron microscopic studies and was also used as biochemical control. The electron microscopic autoradiograms obtained showed the labelled structure of RNA, sRNA and rRNA. The incorporation of labelled purine rings was confirmed in all three RNA types by the radioactivity, which was also proved in nucleotides after hydrolysation of RNA fractions. The results were compared with RNA isolated from tissue cultivated on a non-radiactive medium.

Received: November 11, 1969; Published: September 1, 1970  Show citation

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Volfová, A., Friedrich, A., & Chvojka, L. (1970). Electron microscopic autoradiographic study of RNA isolated from apple-tree callus tissue labelled with 6-Benzylaminopurine-8-C14. Biologia plantarum, 12(5), 327-331. doi: 10.1007/BF02922186
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