biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 13:110-121, 1971 | DOI: 10.1007/BF02936827

Histogenesis and localization of non-specific esterase in root tip

K. Beneš1
1 Institute of Experimental Botany, Czechoslovak Academy of Science, Praha

A procedure was developed for satisfactory freeze-sectioning of root tips. The use of Ca formol-fixed material kept and frozen in Holt's syrup is recommended. The existence and different localization of 2 fractions of non-specific esterase was verified in root tips ofVicia faba. The same results were revealed in fixed and unfixed material. The dynamics ofin situ reaction was followed with respect to optimal incubation time. The results with substrates of different chain length support the existence of 2 fraction of the studied enzyme, none of which, concerning substrate specificity, is a lipase. It follows from the present studies inVicia faba and other species (Cucurbita pepo, Lupinus albus, Pisum sativum Zea mays), that non-specific esterase localization is not directly given by histogenesis.

Received: April 16, 1970; Published: March 1, 1971  Show citation

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Beneš, K. (1971). Histogenesis and localization of non-specific esterase in root tip. Biologia plantarum, 13(2), 110-121. doi: 10.1007/BF02936827
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