biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 13:224-233, 1971 | DOI: 10.1007/BF03332294

A quantitative in vitro study of non-specific esterase in carrot taproot explants using a simultaneous azocoupling method

Ludmila Bedrníková1,2, K. Beneš1,2
1 Institute of Experimental Botany, Czechoslovak Academy of Science, Praha, Czechoslovakia
2 Praha 6 - Vokovice, Czechoslovakia

A simultaneous azocoupling reaction used for in situ localization of non-specific esterase may be applied even for quantitative in vitro measurements. The developed standard procedure was verified with regard to substrate concentration, inhibitory effect of diazonium salt, incubation time, stability of resulting dye, relation between the amount of dye and the amount of material. On HW medium, non-specific esterase activity increases considerably both in phloem and xylem explants from Daucus carota L. taproot. The increase of enzyme is higher and appears earlier than the increase of fresh weight and dry matter, especially in the explants of phloem.

Received: August 12, 1970; Published: July 1, 1971  Show citation

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Bedrníková, L., & Beneš, K. (1971). A quantitative in vitro study of non-specific esterase in carrot taproot explants using a simultaneous azocoupling method. Biologia plantarum, 13(4), 224-233. doi: 10.1007/BF03332294
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