biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 13:405-407, 1971 | DOI: 10.1007/BF02930932

Gametogenesis of tobacco in vitro

J. Bobek1
1 Institute of Genetics and Plant Breeding, Research Institutes for Plant Production, Praha-Ruzyně

Excised anthers ofNicotiana tabacum L. were culturedin vitro at the stage of pollen tetrads, which proved in our experiments to be the most suitable initial stage for cultivation, up to the stage of mature pollen grains, using Ito and Stern's nutrient medium, or this medium supplemented with uracil. Germinating capacity of the pollen grains formed and the lengths of pollen tubes were quantitatively evaluated.

Received: November 27, 1970; Published: September 1, 1971  Show citation

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Bobek, J. (1971). Gametogenesis of tobacco in vitro. Biologia plantarum, 13(5-6), 405-407. doi: 10.1007/BF02930932
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    References

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