biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 17:214-218, 1975 | DOI: 10.1007/BF02920837

Invaginations in plant cell protoplasts containing mycoplasma-like bodies (MLO)

O. Králík1, J. Brčák1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

MLO containing invaginations were found in protoplasts of phloem parenchyma cells in symptomless young leaves ofRibes houghtonianumJancz. infected with a yellows disease. The invaginations originate between the cell wall and plasmalemma, usually at plasmodesmata, and change apparently into superficial vesicles in the protoplast; they are entirely or partially limited by host plasmalemma. The formations mentioned occur in parenchyma cells which contain normal organelles. Sometimes they are divided by a smooth membrane system enclosing MLO. Besides MLO the invaginations contain in some cases slimy fibrils resembling the P-protein in sieve tubes. The MLO bodies seen in invaginations have usually a diameter of 50-250 nm and their plasmalemma (unit membrane) is identical with the plasmalemma of MLO bodies occurring in sieve tubes. However, only few MLO bodies in invaginations are electron dense, so that they resemble naturally degenerated forms of MLO. Similar MLO containing invaginations were formerly described from some leafhoppers transmitting MLO.

Keywords: parenchyma cells; plasmalemma; P-protein; Ribes houghtonianumJancz.; yellows disease

Received: September 2, 1974; Published: May 1, 1975  Show citation

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Králík, O., & Brčák, J. (1975). Invaginations in plant cell protoplasts containing mycoplasma-like bodies (MLO). Biologia plantarum, 17(3), 214-218. doi: 10.1007/BF02920837
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