Biologia plantarum 17:309-313, 1975 | DOI: 10.1007/BF02921226
Comparison between wheat embryos isolated mechanically and by floating off from organic solvents
- 1 Institute for Botany and Microbiology, Nuclear Research Centre Jülich, West Germany
Wheat embryos floated off from organic solvents have been compared with mechanically isolated embryos. The results of comparative investigations, including germination,in vivo formation of polysomes and efficiency of cell free preparations forin vitro protein synthesis, favour the use of floated embryos for studies of biochemical processes during germination.
Received: October 31, 1974; Published: July 1, 1975 Show citation
References
- Chen, D., Sarid, S., Katchalski, E.: Studies on the nature of messenger RNA in germinating wheat embryos.-Proc. nat. Acad. Sci. USA60: 902-909, 1968.
Go to original source... - Johnston, F. B., Stern, H.: Mass isolation of viable wheat embryos.-Nature179: 160-161, 1957.
Go to original source... - Marcus, A.: Protein synthesis in extracts of wheat embryo.-In:Laskin, A. I., Last, J. A. (ed.): Protein Biosynthesis in Non-bacterial systems. Pp. 127-145. M. Dekker, New York 1972.
- Marcus, A., Efron, D., Weeks, D. P.: The wheat cell-free system.-In:Grossman, L., Moldave, K. (ed.). Methods in Enzymology, Part E. in press, 1973.
Go to original source... - Roberts, E., Mathews, M. B., Bruton, Ch. J.: Tobacco mosaic virus RNA directs the synthesis of a coat protein peptide in a cell-free system from wheat.-J. mol. Biol.80: 733-742, 1973.
Go to original source... - Stutz, E., Noll, H.: Characterization of cytoplasmic and chloroplast polysomes in plants: Evidence for the three classes of ribosomal RNA in nature.-Proc. nat. Acad. Sci. USA57: 774-781, 1967.
Go to original source... - Vanderhoef, L. N., Bohannon, R. F., Key, J. L.: Purification of transfer RNA and studies on aminoacyl-tRNA synthetases from higher plants.-Phytochemistry9: 2291-2304, 1970.
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