biologia plantarum

Biologia plantarum 17:385-391, 1975 | DOI: 10.1007/BF02921046

A kinetic study of phosphorus absorption by excised maize roots from flowing solutions with different phosphorus concentrations

Radmila Čížková-Macůrková², Z. Laštůvka¹

¹ Department of Plant Biology, Faculty of Sciences J. E. Purkyně University, Brno

² Department of Forest Biology, Botanical Institute of the Czechoslovak Academy of Sciences, Brno 3, Czechoslovakia

The effect of two different mechanisms of phosphorus ion transport from the nutrient solution volume to the surface areas of excised maize roots was studied under concentrations ranging from 0.01 mM to 50.0 mM KH₂PO₄. A modified technique of study of kinetic ion absorption was used. In the control series, the roots were placed in absorption solution without flow (the dominant mechanism of ion transport to the roots being diffusion), while in the experimental series the absorption solution was flowing round the roots at a rate of 0.162 cm s^-1 (the dominant mechanism of ion transport to the roots being mass flow). The rate of phosphorus absorption by the roots from flowing solutions was highly significantly increased at all concentrations of absorption solution except for the 50.0 mM KH₂PO₄ concentration. The increase in phosphorus absorption in the case of 50.0 mM KH₂PO₄ concentration was non-significant due to the fact that the high concentration of phosphorus together with the diffusion of phosphorus ions ensured a sufficient supply of phosphorus to the roots, covering the requirement for their uptake.
The results point to the need for an analysis of environmental factors to be carried out in studying ion absorption kinetics, and reveal the inadequacy of methods usually employed in such investigations, in particular with respect to the homogeneity of the nutrient solution in the whole of its volume and especially round the roots.

Received: December 27, 1975; Published: November 1, 1975  Show citation