biologia plantarum

Biologia plantarum 17:443-447, 1975 | DOI: 10.1007/BF02921057

Free nucleotides in the primary root of maize

Viera Finocchiaro¹, J. Kolek¹

¹ Institute of Experimental Biology and Ecology of the Slovak Academy of Sciences, Bratislava

Free nucleotides of the primary root of maize were extracted with 5% HClO₄ and separated on a column of Dowex 1×8 ion exchanger in HCOO^- cycle. A two-step elution gradient (HCOOH, HCOOHNH₄) was used for the elution of the nucleotides. The incorporation of³²P into the nucleotides was followed at different time intervals and also in young and more mature root tissues. The nucleotides AMP, GMP, ADP, GDP, and ATP were identified. Labelled phosphorus was found in ATP after 30 s, in ADP after 3 min, and in AMP after 5 min incubation of the roots. More mature roots (18 days) contained higher amounts of AMP than the young ones (3 days).

Received: June 3, 1975; Published: November 1, 1975  Show citation