biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 18:36-43, 1976 | DOI: 10.1007/BF02922331

Cytochrome oxidase and ascorbic acid oxidase activities in cereal plants

Naděía Růžičková-Skřipská1
1 Department of Plant Biology, Faculty of Science, J. E. Purkyně University, Brno, Czechoslovakia

Cytochrome oxidase and ascorbic acid oxidase activities were investigated in rye, wheat, barley and oat plants. The variations in the activity of both enzymes was followed in the course of the initial 28 days of growth, as well as at the phase of milk ripeness, namely in the cytoplasmic and mitochondrial cell fractions of roots, leaves and spikes.
Both enzymes were active in all measurements. Cytochrome oxidase mostly exhibited a higher activity than ascorbio acid oxidase. The activity of the former enzyme was substantially higher in the mitochondrial fraction of leaves, roots and spikes of the four experimental plants in comparison with the cytoplasmic fraction. On the contrary, the ascorbic acid oxidase activity varied in both cell fractions according to the plant species, organ and growth phase.
The variations in the activity of both enzymes exhibited on the whole a course similar to that of the respiration rate. During the first 14 to 21 days of growth the enzyme activities increased up to the maximum. This was thon followed at first by a rapid, later on by a slow decrease. The course of variations in the enzyme activities was, with certain exceptions, alike in all the four plant species investigated.

Received: October 30, 1974; Published: January 1, 1976  Show citation

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Růžičková-Skřipská, N. (1976). Cytochrome oxidase and ascorbic acid oxidase activities in cereal plants. Biologia plantarum, 18(1), 36-43. doi: 10.1007/BF02922331
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