biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 19:2-9, 1977 | DOI: 10.1007/BF02922478

Studies on protein and RJVA syntheses in activated and temperature-blocked seeds ofAgrostemma githago: the influence of benzylaminopurine

M. Hecker1
1 Sektion Biologie FG Allgemeine Botanik und Pflanzenphysiologie, Universität Greifswald Sektion Bilogie, Ernst-Moritz-Arndt-Universität, Greifswald

Embryos ofAgrostemma githago seeds blocked by higher temperature are suitable objects for the study of the regulation of gen expression independent of transeriptional processes. Germination of afterripened seeds can be prevented by imbibing at 30°C, whereas at 20°C germination will be completed after 30 h of soaking (BORRISS 1956). In temperature-blocked embryos RNA is synthesized with undiminished intensity, whereas DNA and protein syntheses are remarkably reduced. Equal amounts of poly(A)-RNA (probably mRNA), enriched by affinity chromatography on oligo(dT)-ceIlulose columns, are detected in blocked and activated embryos.
We suggest that in blocked embryos not synthesis itself, but the availability of mRNA to polysomal fraction is reduced. Benzylaminopurine is able to break secondary dormancy ofAgrostemma githago seeds. In benzylaminopurine-treated seeds there is only a slight increase of the incorporation of14C-L-leu-cine in protein.

Keywords: seed germination; seed dormancy; cytokinin; control of gene expression; disc electrophoresis; poly(A)-RNA.

Received: January 8, 1976; Published: January 1, 1977  Show citation

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Hecker, M. (1977). Studies on protein and RJVA syntheses in activated and temperature-blocked seeds ofAgrostemma githago: the influence of benzylaminopurine. Biologia plantarum, 19(1), 2-9. doi: 10.1007/BF02922478
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