biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 19:150-152, 1977 | DOI: 10.1007/BF02926756

A macerating ammonium oxalate fixative and schedule for some root meristems

C. B. S. R. Sharma1, B. B. Panda1, R. K. Sahu1
1 Department of Botany Berhampur University, Cytogenetics Laboratory, Berhampur, India

0.25 % ammonium oxalate in a mixture of ethyl alcohol, chloroform, formaldehyde, acetic acid and hydrochloric acid works as a very fast and efficient macerating fixative for the root meristems ofAllium cepa, Allium sativum, Ornithogalum thyrsoides, Pisum sativum andVicia faba. Slicing the tip region of excised meristems hastens spindle poison pretreatment, fixation and staining. Owing to the dissolution of the middle lamella and eliminating a physical barrier to permeability an extremely rapid fixation and staining schedule involving several stains has thus been developed.

Received: June 3, 1976; Published: March 1, 1977  Show citation

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Sharma, C.B.S.R., Panda, B.B., & Sahu, R.K. (1977). A macerating ammonium oxalate fixative and schedule for some root meristems. Biologia plantarum, 19(2), 150-152. doi: 10.1007/BF02926756
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    References

    1. Darlington, C. D., La Cour, L. F.: The Handling of Chromosomes. - Allen and Unwin, London 1969.
    2. Lange, N. A.: Handbook of Chemistry. - Mc Graw Hill Co., New York 1956.
    3. Sharma, A. K., Sharma, A.: Chromosome Technique - Theory and Practice. - Butterworths, London 1972.
    4. Sharma, C. B. S. R., Berera, B. N., Velayudham, N.: A modified schedule for sugar-cane chromosomes. - Curr. Sci.45: 28, 1976.
    5. Wolff, S.: Handling of root tips. - In:Prescott, D. M. (ed): Methods in Cell Physiology. Pp. 215-227. Aoad. Press., New York 1964. Go to original source...

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