biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 20:14-18, 1978 | DOI: 10.1007/BF02922924

The isolation of tissue culture ofPopulus alba L. 'Pyramidalis'

Ví»azoslava Blanáriková1, ©. Karácsonyi1
1 Institute of Chemistry, Slovak Academy od Sciences, Bratislava, Czechoslovakia

Tissue culture was isolated from the stem ofPopulus alba L. 'pyramidalis'. Callus formation was observed since November till March (1974),i.e. till the formation of calluses suitable for further subeultivation. The most vigorous growth was obtained with the callus culture cultivated on the nutrient medium of DIAZ-COLONet al. (1972) on which more than 11 g of fresh matter was produced after 30 d at the end of the first year of cultivation in darkness, with inoculum weight 1.5-1.8 g. A mild decrease in growth rate of the tissue culture was observed after the first year of cultivation. When illuminated, the originally yellow calluses turned green. The morphological and anatomical structure of the callus culture is also described and cell shape and cell size evaluated.

Received: November 23, 1976; Accepted: April 27, 1977; Published: January 1, 1978  Show citation

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Blanáriková, V., & Karácsonyi, ©. (1978). The isolation of tissue culture ofPopulus alba L. 'Pyramidalis'. Biologia plantarum, 20(1), 14-18. doi: 10.1007/BF02922924
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