biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 20:305-308, 1978 | DOI: 10.1007/BF02923317

Karyological features of barley callus tissues culturedin vitro

E. U. Scheunert1, Zlata B. Shamina2, H. Koblitz1
1 Department of Cell and Tissue Culture, Central Institute of Genetics and Plant Cultivars, Academy of Sciences of the GDR, Gatersleben, German Democratic Republic
2 Laboratory of Cell and Tissue Cultures, Timiryazev-Institute of Plant Physiology, Academy of Sciences of the USSR, Moscow, USSR

Long-term callus cultures of two barley cultivars have been investigated at the cellular level. One slowly growing, root forming callus of the barley cv. Bulgarische Nackte consists of cells the majority of which contain the diploid chromosome number. Contrarily, a fast growing callus of the cv. Elgina having no regeneration potency at all, is highly polyploid and includes more than two thirds of aneuploid cells. Tn this callus strain, a great number of multinucleate cells has been found. Some problems are discussed with respect to the relationships between growth rate, karyological stability and regeneration capacity in long-term callus cultures of cereals.

Received: November 9, 1977; Accepted: December 15, 1977; Published: September 1, 1978  Show citation

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Scheunert, E.U., Shamina, Z.B., & Koblitz, H. (1978). Karyological features of barley callus tissues culturedin vitro. Biologia plantarum, 20(5), 305-308. doi: 10.1007/BF02923317
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