biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 21:113-118, 1979 | DOI: 10.1007/BF02909458

Short term cultivation of isolated barley roots and their mitotic activity

Běla Landová1, M. Ondřej1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia

Roots were excised from barley embryos cultivated in the complete liquid nutrient solution and cultivated in the same nutrient solution separately. The excised roots continued their growth but a progressive decrease in the growth rate was observed. There was a considerable short-term drop of the mitotic activity immediately after excision, which was followed by a compensatory increase and then equilibrium was reached 12 h after excision. During the next at least three days, the mitotic index of isolated barley roots varied between 5-6.5%, which is slightly lower than the mitotic index of the root meristems of isolated barley embryos under identical conditions. The mitotic cycle index of isolated barley roots and the size of the root meristem later decreased gradually.

Received: August 8, 1978; Accepted: September 25, 1978; Published: March 1, 1979  Show citation

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Landová, B., & Ondřej, M. (1979). Short term cultivation of isolated barley roots and their mitotic activity. Biologia plantarum, 21(2), 113-118. doi: 10.1007/BF02909458
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