biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 21:119-126, 1979 | DOI: 10.1007/BF02909460

Isolation and characterization of a trypsin inhibitor fromVigna sinensis seeds

J. Xavier-Filho1, Francisca D. N. de Souza1
1 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil

The major trypsin inhibitor ofVigna sinensis cv. seridó was isolated and shown to be devoid of chymotrypsin inhibiting activity. It has a molecular weight of 9800 as determined by gel electrophoresis and a pI of 5.0. The activity of the inhibitor was decreased by treatment with trinitrobenzenesulfonic acid, suggesting that it isa LYS-X type trypsin inhibitor. Selfassociation of the molecule was demonstrated both in 1% sodium dodecylsulfate and inacidic (pH 2.4) conditions.

Received: August 23, 1978; Accepted: September 19, 1978; Published: March 1, 1979  Show citation

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Xavier-Filho, J., & de Souza, F.D.N. (1979). Isolation and characterization of a trypsin inhibitor fromVigna sinensis seeds. Biologia plantarum, 21(2), 119-126. doi: 10.1007/BF02909460
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