Biologia plantarum 21:307-310, 1979 | DOI: 10.1007/BF02902215
Relationships between nitrate level, nitrate reductase activity and anaerobic nitrite production inPisum sativum leaf tissue
- 1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6-Vokovice, Czechoslovakia
Anaerobic nitrite production (thein vivo NO3-R activity) in an incubation medium lacking exogenous nitrate but containing 0.5%n-propanol and 0.1% Triton X-100 showed higher correlation (y - axb) with the level of endogenous nitrate inPisum sativum L. leaves than thein vitro nitrate reductase activity. Thein vivo NO3-R activity correlated well with thein vitro activity up to the 50 ppm NO3-N level of endogenous nitrate. The ratioin vivo: in vitro activity slightly decreased with increasing level of endogenous nitrate in leaf tissue.
Received: December 27, 1978; Accepted: January 22, 1979; Published: July 1, 1979 Show citation
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©krdleta, V., Gaudinová, A., & Nìmcová, M. (1979). Relationships between nitrate level, nitrate reductase activity and anaerobic nitrite production inPisum sativum leaf tissue. Biologia plantarum, 21(4), 307-310. doi: 10.1007/BF02902215
References
- Ferrari, T. E., Yodkr, O. C., Filner, P.: Anaerobic nitrite production by plant cells and tissues: Evidence for two nitrate pools. - Plant Physiol.51: 423-431, 1973.
Go to original source... - Johnson, C. B., Whittington, W. J., Blackwood, G. C.: Nitrate reductase as a possible predictive test of crop yield. - Nature262: 133-134, 1976. Go to original source...
- Jones, R. W., Sheard, R. W.: Condition affecting invivo nitrate reduotase activity in chlorophyllous tissues. - Can. J. Bot.55: 896-901, 1977a. Go to original source...
- Jones, R. W., Sheard, R. W.: Differential effect of irradiance and nutrient nitrate on the relationship ofin vivo andin vitro nitrate reductase assay in chlorophyllous tissuea. - Plant Physiol.59: 535-539, 1977b. Go to original source...
- Sohrader, L. E., Cataldo, D. A., Peterson, D. M., Vogelzang, R. D.: Nitrate reductase and glucoso-6-phosphate-dehydrogenase activities as influenced by leaf ago and addition of protein to extraction media. - Plant Physiol.32: 337-341 1974. Go to original source...
- Streeter, J. G., Bosler, M. E.: Comparison of invitro andin vivo assays for nitrate reduotase in soybean leaves. - Plant Physiol.49: 448-450, 1972. Go to original source...