biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 21:365-371, 1979 | DOI: 10.1007/BF02878235

Poly(A)+ RNA synthesis in germinating pollen ofNicotiana tabacum L

J. Süss1, J. Tupı1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia

Poly(A)+RNA is synthesized during the first hours of pollen germination and is rapidly incorporated into polysomal structures. After a 2-h pulse with uracil-14C, 42% of the transcribed fraction of polysomal RNA is polyadenylated. Following 4 h of germination the amount of the newly-made poly(A)+RNA decreases steadily at the rate of about 14% per h, whereas that of rapidly-labelled poly(A)-RNA continues to grow. Beginning 1 h of cultivation the ratio of poly(A)-/poly(A)+RNA increases exponentially. Similarly as in non-polyadenylated mRNA the main portion of the synthesized polysomal poly(A)+RNA sediments at a rate of 4 to 14 S and its mean size decreases slightly with the time of labelling. RNA isolated from nuclei and cell wall containing pollen tube fraction differed from the polysomal one in higher apeoific radioactivity and the polyadenylated RNA exhibited higher size distribution. The comparison of the results with earlier observations suggests the involvement of poly(A)in mRNA translation in pollen tubes.

Received: April 4, 1979; Accepted: April 18, 1979; Published: September 1, 1979  Show citation

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Süss, J., & Tupı, J. (1979). Poly(A)+ RNA synthesis in germinating pollen ofNicotiana tabacum L. Biologia plantarum, 21(5), 365-371. doi: 10.1007/BF02878235
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    References

    1. Abraham, A. B., Jacob, S. T.: Hydrolysis of poly(A) to adenine nucleotides by purified poly(A) polymerase. -Proc. nat. Acad. Sci. USA75: 2085-2087, 1978. Go to original source...
    2. Adolf, G. R. Swetly, P.: Poly(A) polymerase activity during cell cycle and erythropoietic differentiation in erythroleukemic mouse Spleen cells. - Biochim. biophys. Acta518: 334 to 344, 1978. Go to original source...
    3. Angermann, K., Grundmann, U., Holz, H.: Grössenvergleich der Poly(A)-RNA aus Polysomen und aus Kernen bei der HefeSaccharomyces cerevisiae. - Z. Naturforsch.31C: 85-90, 1976. Go to original source...
    4. Balatková, V., Hrabìtová, E., Tupı, J.: The growth activity and fertilizing capacity of submersely cultivated pollen tubes ofNicotiana tabacum L. - J. exp. Bot.28: 1231-1237, 1977. Go to original source...
    5. Brawerman, G., Mendecki, J., Lee, S. Y.: A procedure for the isolation of mammalian messenger UNA.- Biochemistry11: 637-641, 1972. Go to original source...
    6. Coleman, M. S., Hutton, J. J., Bollum, F. J.: Terminal riboadenylate transferase in human lymphocytes. - Nature248: 407-409, 1974. Go to original source...
    7. Delseny, M., Aspart, L., Guitton, Y.: Effect of the protein synthesis inhibitor cycloheximide on RNA synthesis in radish seedlings. - Biochemie59. 51-57, 1977. Go to original source...
    8. Dworkin, M. B., Infante, A. A.: Relationship between the messenger RNA of polysomes and free ribonucleoprotein particles in the early sea urchin embryo. - Develop. Biol.53: 73 to 90, 1976. Go to original source...
    9. Fromson, D., Verma, D. P. S.: Translation of nonpolyadenylated messenger RNA of sea urchin embryos. - Proc. nat. Acad. Sci. USA73: 148-151, 1976. Go to original source...
    10. Glisin, V., Crvenjakov, R., Byus, C.: Ribonucleic acid isolated by cesium chloride centrifugation.- Biochemistry13: 2633-2637, 1974. Go to original source...
    11. Grierson, D., Covey, S.: Changes in the amount of ribosomal RNA and poly (A)-containing RNA during leaf development. - Planta127: 77-86, 1975. Go to original source...
    12. Jacob, S. T., Rose, K. M., Munro, H. N.: Response of poly(adenylic acid) polymerase in rat liver nuclei and mitochondria to starvation and re-feeding with amino acids. - Biochem. J.158: 161-167, 1976. Go to original source...
    13. Lafleur, G. J., Mascarenhas, J. P.: The dependence of generative cell division inTradescantia pollen tubes on protein and RNA synthesis. - Plant Sci. Lett.12: 251-265, 1978. Go to original source...
    14. Linskens, H. F., Schrauwen, J. A. M., Konings, R. N. H.: Cell-free protein synthesis with polysomes from germinatingPetunia pollen grains. - Planta90: 153-162, 1970. Go to original source...
    15. Mascarenhas, J. P.: The biochemistry of angiosperm pollen development. - Bot. Rev.41: 259 to 314, 1975. Go to original source...
    16. Mascarenhas, J. P., Bell, E.: Protein synthesis during germination of pollen: studies on polyribosome formation. -Biochim. biophys. Acta179: 199-203, 1969. Go to original source...
    17. Perry, R. P., La Torre, J., Kelley, D. E., Greenberg, J. R.: On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. - Biochim. biophys. Acta262: 220-226, 1972. Go to original source...
    18. Ragg, H., Schröder, J., Hahlbrock, K.: Translation of poly(A)-containing and poly(A)-free messenger RNA for phenylalanine ammonia-lyase, a plant-specific protein, in a reticulocyte lysate. - Biochim. biophys. Acta474: 226-233, 1977. Go to original source...
    19. Rhoads, R. E.: Ovalbumin messenger RNA. - J. biol. Chem.250: 8088-8097, 1975. Go to original source...
    20. Rodriguez-Pousada, C., Hayes, D. H.: Poly(A)-containing RNA inTetrahymena pyriformis. - Eur. J. Biochem.71: 117-124, 1976. Go to original source...
    21. Sun, S.-M. M., Buchbinder, B. U., Hall, T. C.: Cell-free synthesis of the major storage protein of the bean,Phaseolus vulgaris L. - Plant Physiol.56: 780-785, 1975. Go to original source...
    22. Taylor, J. M., Tse, T. P. H.: Isolation of rat liver albumin messenger RNA. - J. biol. Chem.251: 7461-7467, 1976. Go to original source...
    23. Tobin, E. M., Klein, A. O.: Isolation and translation of plant messenger RNA. - Plant Physiol.56: 88-92, 1975. Go to original source...
    24. Tupı, J.: RNA synthesis and polysome formation in pollen tubes. - Biol. Plant.19: 300-308, 1977. Go to original source...
    25. Tupı, J., Hrabìtová, E., Balatková, V.: The release of RNA from pollen tubes. - In:Linskens, H. F. (ed.): Fertilization in Higher Plants. Pp. 145-150. North-Holland Publ. Co., Amsterdam 1974.
    26. Tupı, J., Hrabìtová, E., Balatková, V.: A simple rapid method of determining pollen tube growth in mass culture. - Plant Sci. Lett.9: 285-290, 1977a. Go to original source...
    27. Tupı, J., Hrabìtová, E., Balatková, V.: Evidence for ribosomal RNA synthesis in pollen tubes in culture. - Biol. Plant.19: 226-230, 1977b. Go to original source...
    28. Verma, D. P. S., Maclachlan, G. A., Byrne, H., Ewings, D.: Regulation andin vitro translation of messenger RNA for cellulase from auxin-treated pea epicotyls. - J. biol. Chem.250: 1019 to 1026, 1975. Go to original source...

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