biologia plantarum

Biologia plantarum 22:57-64, 1980 | DOI: 10.1007/BF02878128

Utilization of exogenous sugars by excised maize embryos in culture

Kornélia Burghardtová¹, J. Tupý²

¹ Institute of Botany, Slovak Academy of Sciences, Bratislava

² Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia

Sucrose was markedly superior to fructose and glucose in promoting growth of plantlets from immature maize embryos. The elongation of roots is shown to be more sucrose dependent than that of shoots. On the other hand, the exogenous sucrose was less effective than fructose as substrate for carbohydrate catabolism and for the synthesis of alcohol-insoluble compounds at the beginning of embryo cultivation. The absorbed fructose was found to be rapidly converted to sucrose and the level of endogenous sucrose derived from sugar supplied to the medium was higher in fructosethan in sucrose-fed embryos. The preferential utilization of fructose over sucrose, however, declined with the progress of germination which may be related to the decrease in proportion of scutellum in total mass and physiological activity of the embryo.

Received: August 9, 1979; Accepted: September 7, 1979; Published: January 1, 1980  Show citation