biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 22:255, 1980 | DOI: 10.1007/BF02892768

Chlorophyllase in citrus leaves. Kinetic aspects of the reaction

A. L. García1, L. Galindo1, S. Navarro1
1 Department of Agricultural Chemistry, Faculty of Sciences, University of Murcia, Spain

The reaction: Chlorophyll + Enzyme → Chlorophyllide + Phytol follows a first order kinetics with regard to the quantity of enzyme, when it is saturated by substrate. Km and Vm were determined from the average reaction rates for the substrates: Chlorophylla andb, pheophytina andb, methylchlorophyllidea and methylpheophorbide a: The lowest Km corresponded to chlorophylla and the highest Vm to methylpheophorbidea. For a substrate of chlorophyll (a + b), Km and Vm were determined also using the initial reaction rates. "Enzyme efficiency" was calculated using both methods of determination. The reaction products partially inhibit the hydrolytic process.

Received: June 19, 1979; Accepted: October 2, 1979; Published: July 1, 1980  Show citation

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García, A.L., Galindo, L., & Navarro, S. (1980). Chlorophyllase in citrus leaves. Kinetic aspects of the reaction. Biologia plantarum, 22(4), 255. doi: 10.1007/BF02892768
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