Biologia plantarum 22:303, 1980 | DOI: 10.1007/BF02892778
Chromosomal instabilities in callus tissue from haploid barley (Hordeum vulgare L.)
- 1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha
- 2 Experimental Station Olomouc, Olomouc, Czechoslovakia
Callus culture was derived from haploid barley embryos after crossing withHordeum bulbosum. The callus tissue is cytologically heterogeneous, containing haploid, diploid and polyploid cells. Aneuploidy and karyokinetic irregularities were also observed. Some problems of chromosomal instabilities in plant tissue cultures are discussed.
Received: September 13, 1979; Accepted: November 29, 1979; Published: July 1, 1980 Show citation
References
- D'Amato, F.: Cytogenetics of differentiation in tissue and oell oultures. -In:Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Pp. 343-357. Springer Verlag, Berlin-Heidelberg-New York 1977.
- Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirement of suspension cultures of soybean root cells. -Exp. Cell Res.50: 151-158, 1968.
Go to original source... - Jensen, C. J.: Monoploid production by chromosome elimination. -In:Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture. Pp. 299-330, Springer Verlag, Berlin-Heidelberg-New York 1977.
- Novák, F. J.: The changes of karyotype in callus cultures ofAllium sativum L. -Caryologia27: 45-54, 1974.
Go to original source... - Novák, F. J., Adamusová, M.: [The induction of callogenesis in thein vitro culture ofHordeum vulgare L]. In Czech. -In: Kolokviumo rastl. expl. kult., Stupy pri Pezinku. Pp. 39-56, Univ. Komenského, Bratislava 1974.
Go to original source... - Novák, F. J., Minařík, F., Bouma, J., Ohnoutková, L.: Production of haploid barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) in interspecific hybridization withHordeum bulbosum L. -In:Novák, F. J. (ed.) Use of Tissue Cultures in Plant Breeding. Pp. 427-439. Czech. Acad. Sci., Inst. Exp. Bot., Praha 1977.
- Novák, F. J., Vyskot, B.: Karyology of callus cultures derived fromNicotiana tabacum L. haploids and ploidy of regenerants. -Z. Pflanzenzücht.75: 62-70, 1975.
- Saalbach, G., Koblitz, H.: Karyological instabilities in callus cultures from haploid barley plants. -Biochem. Physiol. Pflanzen171: 469-473, 1977.
Go to original source... - Sacristán, M. D.: Karyotypic changes in callus cultures from haploid and diploid plants ofCrepis capillaris (L.). Wallr. -Chromosoma33: 273-283, 1971.
Go to original source... - Scheunert, E. U., Shamina, Z. B., Koblitz, H.: Studies on barley calluses culturedin vitro: I. Establishment, maintenance and growth of two different tissue strains. -Plant Sci. Lett.10: 313-318, 1977.
Go to original source... - Scheunert, E. U., Shamina, Z. B., Koblitz, H.: Karyological features of barley oallua tissue culturedin vitro. -Biol. Plant.20: 305-308, 1978.
Go to original source... - Shamina, Z. B., Scheunert, E. U., Koblitz, H.: Studies on barley calluses culturedin vitro: II. Cytological and morphological features of barley callus cultures. -Plant Sci. Lett.13: 177-184, 1978.
Go to original source... - Sunderland, N.: Nuclear cytology. -In:Street, H. E. (ed.): Plant Tissue and Cell Culture. Pp. 161-190. Blackwell Sci. Publ., Oxford 1973.



