biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 24:188-194, 1982 | DOI: 10.1007/BF02883661

Peroxidase activity and isoenzyme patterns in wheat during ontogenesis

Hana Bartošová1, Ivana Macháčková1, Z. Zmrhal1
1 Department of Plant Nutrition, Institute of Crop Production, Praha 6, Czechoslovakia

Protein content, total and specific peroxidase activity and isoperoxidase patterns were determined in crude protein preparations from individual parts of field-grown wheat (Triticum aestivum L., cv. Jubilar). Protein content in roots, leaves, and stalks increased at the beginning of ontogenesis and then decreased from 6th, 9th, and 10th development phase (according to Feekes), respectively. Steady increase of the protein content in the ears was observed.
Highest peroxidase activity was found in the roots; it diminished from the onset of ontogenesis till maturity of the plants. In the leaves and stalks a slight decrease of peroxidase activity till the 10th development phase and then an increase till maturity was found. The ears exhibited a gradual increase of peroxidase activity. The course of specific peroxidase activity was found to be very similar to that of total activity.
Isoperoxidase patterns did not change significantly. In the leaves, a decrease of activity of C4 and C5 isoperoxidases was recorded. In the stalks, C l isoenzyme emerged at the end of ontogenesis. A gradual increase of A1 and A5 isoperoxidase intensity took place both in the leaves and stalks.

Received: June 15, 1981; Accepted: September 11, 1981; Published: May 1, 1982  Show citation

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Bartošová, H., Macháčková, I., & Zmrhal, Z. (1982). Peroxidase activity and isoenzyme patterns in wheat during ontogenesis. Biologia plantarum, 24(3), 188-194. doi: 10.1007/BF02883661
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