biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 24:394, 1982 | DOI: 10.1007/BF02909110

Comparative studies on the electrophoretic patterns of acid-soluble chromosomal proteins duringZea mays early stages of embryo germination and root cell differentiation

Elesa Marinova1, Stanka Koleva1
1 M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

Acid-soluble chromosomal proteins were extracted from purified nuclei, isolated from 3 - 4 h, 12 -14 h and 24 -26 h maize embryos, as well as from nuclei isolated from meris-tematic, elongating and differentiated cells, from 2 and 3-day-oldZea mays seedlings primary roots.
Using polyacrylamide gel electrophoresis (urea-acetic acid in the cylindrical gels and slab-SDS-electrophoresis), it was established that germination and root cell differentiation induced changes in some nuclear acid-soluble chromosomal proteins, especially in histone H1.
The results of proteins belonging to the high-mobility group proteins (HMG) and some other acid-soluble proteins with unknown nature for the plants, based on the electrophoretic mobility, were discussed.

Received: October 20, 1981; Accepted: March 9, 1982; Published: September 1, 1982  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Marinova, E., & Koleva, S. (1982). Comparative studies on the electrophoretic patterns of acid-soluble chromosomal proteins duringZea mays early stages of embryo germination and root cell differentiation. Biologia plantarum, 24(5), 394. doi: 10.1007/BF02909110
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Gofshtein, L. V.: [Histories of plant origin in connection with evolution from prokaryotes and eukaryotes.] In Russ. -Biokhimiya43: 947-958, 1978.
    2. Grellet, F., Delseny, M., Guition, J.: Histone content of germinating pea embryo chromatin decreases as DNA replicates. - Nature207: 724-726, 1977. Go to original source...
    3. Jenson, J. C., Chin-Lin, P., Gerber-Jenson, B., Litman, G. W.: Structurally unique basic protein co-extracted with histones from calf thymus chromatin. - Proc. nat. Acad. Sci. USA77: 1389-1393, 1980. Go to original source...
    4. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature227: 680-683, 1970. Go to original source...
    5. Marinova, E. I., Koleva, S. T., Varadinova, S. Ts., Koteva, N. B.: [Root cell growth induced changes in nuclear and chromatin proteins ofZea mays L.] In Russ. - In:Georgiev, G., Bakardjieva, N., Koleva, S. (ed.): Self-Regulation of Plant Metabolism. Pp. 152-159. University Press, Sofia 1981.
    6. Mayer, A. M., Shain, Y.: Control of seed germination. - Annu. Rev. Plant Physiol.25: 167 to 193, 1974. Go to original source...
    7. Muller, A. G., Phillipps, G., Gigot, C.: Properties of condensed chromatin in barley nuclei. -Planta149: 69-77, 1980. Go to original source...
    8. Murray, M. G., Key, J. L.: 2,4-Dichlorophenoxyacetic acid-enhanced phosphorylation of soybean nuclear proteins. -Plant Physiol.61: 190- 198, 1978. Go to original source...
    9. Nadeau, P., Pallotta, D., Lafontaine, J.-G.: Electrophoretic study of plant histones; com- parison with vertebrate histones. - Arch. Biochem. Biophys.161: 171-177, 1974. Go to original source...
    10. Panyim, S. R., Chalkley, R.: High resolution acrylamide gel electrophoresis of histones. - Arch. Biochem. Biophys.130: 337-346, 1969. Go to original source...
    11. Rindt, K. P., Nover, L.: Chromatin structure and function. - Biol. Zentralbl.99: 641-673, 1980.
    12. Rodriguez, Y. A., Brandt, W.: Plant histone 2 from wheat germ a family of histone H2A variants. Partial amino acid sequences. - Biochem. biophys. Acta578: 196-206, 1979. Go to original source...
    13. Shannon, M. C., Daniel, R. G.: Histories and histone-DNA ratios in diploid and polyploid cot- tons. - Growth43: 252-262, 1979.
    14. Spiker, S.: Molecular weight histone variants in wheat embryo chromatin. - Plant Physiol.65: 80-83, 1980.
    15. Spiker, S., Mardian, J. K. W., Isenberg, I.: Chromosomal HMG proteins occur in three eukaryotic kingdoms. - Biochem. biophys. Res. Commun.82: 129-135, 1978. Go to original source...
    16. Sugita, M., Nakanishi, Y. H., Sasaki, K.: Electron microscopic study on the chromatin ex- tracted from wheat germ and seedlings. - Proc. Japan Acad.56: 211-214, 1980. Go to original source...
    17. Sugita, M., Yoshida, K., Sasaki, K.: Germination induced changes in chromosomal proteins of spring and winter wheat embryos. - Plant Physiol.64: 780-785, 1979. Go to original source...
    18. Towill, L. E., Nooden, L. D.: An electrophoretic analysis of the acid soluble chromosomal proteins from different organs of maize seedlings. - Plant Cell Physiol.14: 851-863, 1973.
    19. Yoshida, K., Sasaki, K.: Changes of template activity of proteins of chromatin during wheat germination. -Plant Physiol.59: 497-501, 1977. Go to original source...
    20. Yoshida, K., Sugita, M., Sasaki, K.: Involvement of nonhistone chromosomal proteins in transcriptional activity of chromatin during wheat germination. - Plant Physiol.63: 1016 to 1021, 1979. Go to original source...

    Return to the content