biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 25:209, 1983 | DOI: 10.1007/BF02902105

Cholinesterase activity in some species of theAllium genus

Vera Hadačová1, Květa Vacková1, Eva Klozová2, M. Kutáček1, Květa Pitterová2
1 Czechoslovak Academy of Sciences, Institute of Experimental Botany, Praha 6, Ke dvoru 16/15, Czechoslovakia
2 Czechoslovak Academy of Sciences, Institute of Experimental Botany, Praha 6, Na Pernikářce 59, Czechoslovakia

In partly purified protein complexes obtained from 22 species of theAllium genus and 6 cultivars ofAllium cepa the activity of cholinesterases was detected and measured using the method of Ellman et al. The degree of its inhibition with 10-4 M neostigmine was also tested. It was found that the activity of cholinesterase differed in individual species up to two hundred times, while the differences in the inhibitory activity of 10-4 M neostigmine occurred only in a few cases. Individual sections and cultivars could not be characterized on the basis of the differences in the activities of the cholinesterases. Of all the sections that ofPhyllodolon shows the highest average activity. In the case of the tested cultivars distinctly the lowest activity was observed in cv. Kastická. The values of the enzymatic activity measured by Ellman's method in this plant material include the activity of specific and unspecific cholinesterases and the part uninhibitable by neostigmine.

Received: May 19, 1982; Accepted: October 21, 1982; Published: May 1, 1983  Show citation

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Hadačová, V., Vacková, K., Klozová, E., Kutáček, M., & Pitterová, K. (1983). Cholinesterase activity in some species of theAllium genus. Biologia plantarum, 25(3), 209. doi: 10.1007/BF02902105
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