biologia plantarum

Biologia plantarum 25:343-348, 1983 | DOI: 10.1007/BF02878278

Lectins from the genusArtocarpus

E. de Azevedo Moreira¹, J. T. A. de Oliveira¹

¹ Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, CE-Brazil

The lectins from the seeds of two species of the genusArtocarpus (A. integrifolia L. andA. incisa L.) were isolated by affinity chromatography on an AB (+) human stoma column, and compared by physicochemical and immunochemical methods. Although with marked differences in the hemagglutinating activity, the elution pattern of the albummic and globulinic fractions from both seeds were comparable by affinity chromatography on a human-stroma column. The two isolectin families presented many similarities. All the lectins presented the same subunit pattern by SDS-polyacrylamide gel electrpphoresia, were recognised by both rabbit antisera prepared against the crude extracts from the two seeds, and presented fused rockets when electrophoresed in agarose gel containing one or the other antiserum.

Received: September 14, 1982; Accepted: December 1, 1982; Published: September 1, 1983  Show citation