biologia plantarum

Biologia plantarum 26:16-21, 1984 | DOI: 10.1007/BF02880419

An improved system of subjecting plants to water stress

P. N. Singh¹, R. Prasad¹, M. Salim¹, A. Sharga¹

¹ Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad, India

An improved system of plant cultivation at stable and specific levels of polyethyleneglycol (PEG, mol. mass 1400-1600) - induced water stress has been described. To set up this system a perforated tubular glass vessel containing soil to support seedling growth was wrapped externally first with a layer of macroporous silica gel-G and then with three layers of a dialysis membrane of a lower exclusion limit (2000 mol. mass). Effects of 8 days of PEG - induced stress have been studied on uptake and translocation of N and P and growth of barley (Hordeum vulgare L. cv. KN 16) seedlings. Some of the noteworthy improvements of the system were exelusion of PEG from the plant consequent upon use of silica gel-membrane combination, shorter time (2 days) for the soil-plant-air continuum to attain steady state, and stability of the plant water potential over a period of a few days.

Received: January 3, 1983; Accepted: May 24, 1983; Published: January 1, 1984  Show citation