Biologia plantarum 26:113, 1984 | DOI: 10.1007/BF02902274
Lectin fromCanavalia brasiliensis (MART.). isolation, characterization and behavior during germination
- 1 Departamento de Bioquímica e Biologia Molecular, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
A lectin was isolated fromCanavalia brasiliensis Mart. seeds by combining solubility fractionation with affinity chromatography on Sephadex G-50. The lectin showed a carbohydrate specificity for D-mannose (D-glucose) binding and a requirement for Ca2+ and Mn2+. All the hemagglutinating activity was found in the cotyledons and the presence of the lectin was followed during the first 15 days of plant germination, through the activity against rabbit erythrocytes, the presence of the "lectin peak" in Sephadex G-50 affinity chromatography, presence of the "lectin bands" in SDS-polyacrylamide gel electrophoresis and the "lectin arcs and rockets" in immunoelectrophoresis in agarose gel. On application of all these methods the lectin showed a differentiated metabolism, disappearing more slowly than the other high molecular weight proteins of the seed.
Received: June 1, 1983; Accepted: September 6, 1983; Published: March 1, 1984 Show citation
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