biologia plantarum

Biologia plantarum 26:181-188, 1984 | DOI: 10.1007/BF02895046

Hormonal autonomy ofArabidopsis thaliana calli

M. Ondřej^1,2, K. Pešina^1,2, Daniela Pavingerová^1,2

¹ Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

² Na sádkách 702, Czechoslovakia

The growth ofArabidopsis thaliana calli on media without growth regulators was studied Calli under study showed autonomy for cytokmms independently whether cultivated on the light or m the dark When cultivated in intense light, they were able to grow, either transiently or permanently, on the medium without any growth regulators In the dark, they were strictly dependent on 2,4 D m the medium Both the intensity of growth and the duration of the transient growth on the medium without growth regulators m the light decreased with the duration of the previous cultivation on the medium with growth regulators The intensity of growth on the medium without grow th regulators was best and the growth was permanent m the callus clone of spontaneous origin which was never treated by growth regulators The degree of chromosomal variability (assessed as the number of chromocentres) m this callus line was lower than that in calli induced on media with growth regulators and then transferred onto medium without growth regulatois.

Received: June 6, 1983; Accepted: October 14, 1983; Published: May 1, 1984  Show citation