biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 26:401, 1984 | DOI: 10.1007/BF02909585

Purification of cytokinins on a polyvinylpyrrolidone column followed by analysis on a reversedphase C18ODS HPLC system

J. Challice1
1 Long Ashton Research Station, University of Bristol, England

It has been found that cytokinins, as a class, can be separated from co-occurring phenolics by column chromatography using polyvinylpyrrolidone (PVP) with methanol as eluant. Subsequent fractionation of the cytokinins can then be achieved by HPLC on a C18ODS reversed phase system using methanol: water (60: 40) as the mobile phase. The system shows considerable promise as an extremely mild separation process and has been used to separate two unknown cytokinins from seedlings ofHordeum vulgare cv. Steptoe.

Received: January 26, 1984; Accepted: March 6, 1984; Published: November 1, 1984  Show citation

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Challice, J. (1984). Purification of cytokinins on a polyvinylpyrrolidone column followed by analysis on a reversedphase C18ODS HPLC system. Biologia plantarum, 26(6), 401. doi: 10.1007/BF02909585
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    References

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