biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 27:105-109, 1985 | DOI: 10.1007/BF02902143

Auxin binding site in tobacco cells

Helen M. Bailey1, R. D. J. Barker1, K. R. Libbenga2, P. C. G. van der Linde2, A. M. Mennes2, M. C. Elliott1
1 School of Life Sciences, Leicester Polytechnic, Leicester, UK
2 Botanisch Laboratorium, Rijksuniversiteit Leiden, VJ Leiden, The Netherlands

A specific, high affinity, IAA binding site was demonstrated in both a cytosolic fraction, and in isolated nuclei, fromNicotiana tabacum cv. Wisconsin No. 38 cells grown in suspension culture. The amount of the binding site detected in both these fractions changed during the culture cycle according to a strict pattern. The molecular mass of the binding site was estimated by gel filtration to be approximately 175 000 and it appears to be a protein. When partially purified by affinity chromatography and allowed to pre-incubate with IAA, the site had a significant stimulatory effect on total RNA synthesis, as measured by a cell-free assay system. Unpurified extracts had no such effects. The system behaves rather like the steroid hormone-receptor system in animals.

Published: March 1, 1985  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bailey, H.M., Barker, R.D.J., Libbenga, K.R., van der Linde, P.C.G., Mennes, A.M., & Elliott, M.C. (1985). Auxin binding site in tobacco cells. Biologia plantarum, 27(2-3), 105-109. doi: 10.1007/BF02902143
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bailey, H. M.: Receptors for plant growth substances. - Ph.D Thesis, Leicester Polytechnic, U.K. (C.N.A.A.) 1983.
    2. Bevan, M., Northcote, D.H.: Some rapid effects of synthetic auxins on mRNA levels in cultured plant cells.- Planta152: 32-35, 1981. Go to original source...
    3. Bogers, R. J., Kulescha, Z., Quint, A., van Vliet, Th. B.: The presence of a soluble auxin receptor and the metabolism of 3-indole-acetic acid in tobacco-pith expiants. - Plant Sci. Lett.19: 311-317, 1980. Go to original source...
    4. Bouman, H., Mennes, A. M., Libbenga, K. R.: Transcription in nuclei isolated from tobacco tissues.- FEBS Lett.101: 369-372, 1979. Go to original source...
    5. Farrimond, J. A., Elliott, M. C., Clack, D.W.: Auxin structure/activity relationships: aryloxyacetic acids. - Phytochemistry20: 1185-1190, 1981. Go to original source...
    6. Libbenga, K. R.: Hormone receptors in plants. - In:Thorpe, T. A. (ed.): Frontiers of Plant Tissue Culture 1978. Pp. 325-333. I.A.P.T.C., Calgary 1978.
    7. Linsmaier, E. M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. - Physiol. Plant.18: 100-127, 1965. Go to original source...
    8. Mennes, A. M., Bouman, H., van der Berg, M. P. M.,Libbenga, K. R.: RNA-synthesis in isolated tobacco-callus nuclei, and the influence of phytohormones. - Plant Sci. Lett.13: 229-239, 1978. Go to original source...
    9. O'Malley, B. W., Schrader, W. T.: The receptors of steroid hormones. - Sci. Amer.234: 32-43, 1976. Go to original source...
    10. Oostrom, H., Kulescha, Z., vax Vliet, Th. B.,Libbenga, K. R.: Characterization of a cytoplasmic auxin receptor from tobacco-pith callus. - Planta149: 44-47, 1980. Go to original source...
    11. Scatchard, G.: The attraction of proteins for small molecules and ions. - Ann. N. Y. Acad. Sci.57: 660-672, 1949. Go to original source...
    12. Theologis, A., Ray, P. M.: Early auxin-regulated polyadenylated mRNA-sequences in peastem tissue. - Proc. nat. Acad. Sci. USA79: 418-421, 1982. Go to original source...
    13. Van der Linde, P. C. G.,Bouman, H., Mennes, A. M., Libbenga, K. R.: A soluble auxinbinding protein from cultured tobacco tissues stimulates RNA synthesisin vitro. - Planta160: 102-108, 1984. Go to original source...
    14. Zurfluh, L. L., Guilfoyle, T. J.: Auxin-induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyl. - Plant Physiol.69: 332 to 337, 1982. Go to original source...

    Return to the content