biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 27:209, 1985 | DOI: 10.1007/BF02902162

Sensitized bean first internode bioassay for auxins and brassinosteroids

M. Strnad1, M. Kamínek1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia

The bean first internode bioassay ofMeudt andBennett (Physiol. Plant.44: 422-428, 1978) is based on the curvature of bean internode sections after unilateral application of test material near to their base. The test was also used for estimation of biological activity of brassinosteroids which enhance the effect of auxin. Sensitivity of the assay to both IAA and 24-epibrassinolide was increased 1000 and 100 times, respectively, when internode sections were incubated in morphologically inversed position. Under these conditions test material was applied to the young auxin-sensitive morphologically apical internode tissues and auxin was basipetally translocated to the zone of curvature. Using this bioassay as little as 10 fmol of IAA and 1 pmol of 24-epibrassinolide can be estimated.

Published: March 1, 1985  Show citation

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Strnad, M., & Kamínek, M. (1985). Sensitized bean first internode bioassay for auxins and brassinosteroids. Biologia plantarum, 27(2-3), 209. doi: 10.1007/BF02902162
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