Biologia plantarum 27:286-291, 1985 | DOI: 10.1007/BF02879864
Hormonal control of morphogenesis in leaf segments ofCentaurium erythraea
- 1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6
- 2 Botanical Institute, Czechoslovak Academy of Sciences, Průhonice, Czechoslovakia
Transversally cut leaf segments ofCentaurium erythraea were cultivated on MS medium. Effects of segment polarity, IAA and sucrose concentrations, light and medium volume on morphogenesis were studied. Shoots generally formed at lower (1.3 × 10-6 mol 1-1) IAA concentrations than roots and callus (1.1 × 10-5-3.4 × 10-5 mol 1-1). Leaf polarity strongly modified the effect of IAA concentration, shifting organogenesis at the segment base toward decreased IAA concentrations as compared with segment apex. Light, sucrose concentration above 3 % and high medium volume changed IAA dependence of morphogenesis in various ways and generally suppressed segment polarity.
Published: July 1, 1985 Show citation
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