biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 27:465-467, 1985 | DOI: 10.1007/BF02894720

Crown gall tumors inCentaurium

Jana Dusbábková1, J. Nečásek1, K. Pešina1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, České Budějoviee, Czechoslovakia

Agrobacterium tumefaciens C58M (a nopaline strain) was used for induction of crown gall tumors inCentaurium umbellatum Gilib. The formation of tumors is very rare. After elimination of bacteria the tumor tissues grow in the light on R3B medium without growth regulators. They are positive in nopaline synthase.

Received: October 8, 1984; Accepted: December 3, 1984; Published: November 1, 1985  Show citation

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Dusbábková, J., Nečásek, J., & Pešina, K. (1985). Crown gall tumors inCentaurium. Biologia plantarum27(6), 465-467. doi: 10.1007/BF02894720
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References

  1. De Cleene, M., De Ley, J.: The host range of crown gall. - Bot. Rev.42: 388-466, 1976 Go to original source...
  2. Chilton, M.D., Drummond, M. H., Merlo, D. J., Sciaky, D., Montoya, A. L., Gordon, M. P. Nester, E. W.: The molecular basis of crown gall tumorigenesis. - Cell11: 263-271, 1977 Go to original source...
  3. Hooykaas, P. J. J.: Plasmid genes essential for the interactions ofAgrobacteria andRhizobia with plant cells. - In:Pühler, A. (ed.): Molecular Genetics of the Bacteria-Plant Inter- actions..229-239. Springer-Verlag, Berlin-Heidelberg-New York-Tokyo 1983. Go to original source...
  4. Langley, R. A., Kado, C. T.: Studies inAgrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine and relationship ofA. tumefaciens mutants to crown gall tumor induction. - Mutation Res.14: 277-286, 1972. Go to original source...
  5. Meredith, C. P.: Shoot development in established callus cultures of cultivated tomato (Lyco- persicon esculentum Mill). -Z. Pflanzenphysiol.95: 405-411, 1979. Go to original source...
  6. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. -Physiol. Plant.15: 473-497, 1962. Go to original source...
  7. Negrutiu, I., Beeftink, F., Jacobs, M.:Arabidopsis thaliana as a model system in somatic cell genetics. I. Cell and tissue culture. - Plant Sci. Lett.5: 293-304, 1975. Go to original source...
  8. Otten, L., Schilperoort, R. A.: A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. - Biochim. biophys. Acta527: 497-500, 1978. Go to original source...
  9. Tamm, B.: Experimentelle Untersuchungen über die Verbreitung des bakteriellen Pflanzen- krebses und das Auftreten von Sekundärtumoren. - Arch. Mikrobiol.20: 273-292, 1954. Go to original source...