biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 28:152-155, 1986 | DOI: 10.1007/BF02885218

Differentiation ofPetunia hybrida tissues transformed byAgrobacterium rhizogenes andAgrobacterium tumefaciens

M. Ondřej1, Růzena Bísková1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na sádkách 702, Czechoslovakia

Petunia hybrida plants were inoculated with differentAgrobacterium rhizogenes andA. tumefaciens strains and developed tumors were further cultivatedin vitro. Transformed flowering plants differentiated from tumors induced byA. rhizogenes strains 8196 and TRIOL Transformed but non-rooted plants developed also from tumors incited byA. tumefaciens T37. Cultures of roots transformed byA. rhizogenes strain 15834 did not show increased incidence of chromosomal aberrations in anaphases in comparison with untransformecl control. Permanent growth of isolated untransformedPetunia roots was not induced by addition of IAA into the medium.

Received: January 14, 1985; Accepted: April 16, 1985; Published: March 1, 1986  Show citation

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Ondřej, M., & Bísková, R. (1986). Differentiation ofPetunia hybrida tissues transformed byAgrobacterium rhizogenes andAgrobacterium tumefaciens. Biologia plantarum, 28(2), 152-155. doi: 10.1007/BF02885218
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