biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 28:265, 1986 | DOI: 10.1007/BF02902290

Binary plant vector based on Ri plasmid and part of T-DNA of the Ti plasmid

M. Ondřej1, Růzena Bísková1, J. Vlasák1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, České Budêjovice, Czechoslovakia

A binary vector system inA. tumefaciens for the introduction of foreign genes into the plant genome was developed. The first component is the Ri plasmid and the second component is a small vector plasmid, replicating inAgrobacterium, which carries the 25 bp terminal sequence of the Ti plasmid, theNos gene as the selectable marker and neighbouring sequences of the Ti plasmid. Functions necessary for integration are providedin trans by the virulence region of the Ri plasmid. Transformed cells are selected on the basis of hairy root tumor proliferation and agropine synthesis. They also showNos activity coded by the gene on the small part of Ti T-DNA.

Received: June 19, 1985; Accepted: September 25, 1985; Published: July 1, 1986  Show citation

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Ondřej, M., Bísková, R., & Vlasák, J. (1986). Binary plant vector based on Ri plasmid and part of T-DNA of the Ti plasmid. Biologia plantarum, 28(4), 265. doi: 10.1007/BF02902290
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