biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 30:179-184, 1988 | DOI: 10.1007/BF02878753

Some factors affecting root formation onin vitro regenerated pea shoots

Marie Kubaláková1, Eva Tejklová2, M. Griga2
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Sokolovská 6, Czechoslovakia
2 OSEVA Research and Breeding Institute of Technical Crops and Legumes, ©umperk-Temenice, Czechoslovakia

The rooting ability of 2 cm long shoots ofPisum sativum L., derived from differentin vitro shoot-tip cultures in two pea cultivars Bohatýr and Kleine Rheinländerin was evaluated. In three mutually independent experiments the full and half-strength Murashige-Skoog medium (containing full or half concentration of macro and microelements), with sucrose concentrations 10-30 g l-1, and with various NAA and IAA combinations, was tested. The variant with half concentration of macro- and microelements, supplemented with 30 g l1 sucrose, and with growth regulators in the quantity of 1 μM proved optimum.

Received: July 24, 1987; Accepted: December 9, 1987; Published: May 1, 1988  Show citation

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Kubaláková, M., Tejklová, E., & Griga, M. (1988). Some factors affecting root formation onin vitro regenerated pea shoots. Biologia plantarum, 30(3), 179-184. doi: 10.1007/BF02878753
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