biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 30:247-251, 1988 | DOI: 10.1007/BF02878202

Karyotype stability in long-term callus derived plants ofCrepis tectorum L.

J. Sengupta1, S. Jha1, S. Sen1
1 Centre of Advanced Study, Department of Botany, University of Calcutta, Calcutta, India

The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.

Received: September 3, 1987; Accepted: December 28, 1987; Published: July 1, 1988  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sengupta, J., Jha, S., & Sen, S. (1988). Karyotype stability in long-term callus derived plants ofCrepis tectorum L. Biologia plantarum, 30(4), 247-251. doi: 10.1007/BF02878202
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bayliss, M. W.: Chromosomal variation in plant tissues in culture. - In: Vasil, I. K. (ed.): International Review of Cytology. Suppl. IIA. Pp. 113-144. Academic Press, New York 1980.
    2. Clbmbnt, W. M.: Stability of chromosome numbers in tissue cultures of Alfa-alfa (Medicago sativa). - Amer. J. Bot.51: 670, 1964.
    3. D'Amato, P.: Cytogenetics of differentiation in tissue and cell cultures. - In: Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Pp. 343-357. Springer-Verlag, New York 1977.
    4. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473-497, 1962. Go to original source...
    5. Novak, J. F., Dolezel, J.: Karyological and cytophotometric study of callus induction inAllium sativum L. - J. Plant Physiol.118: 421-429, 1985. Go to original source...
    6. Sacristan, M. D.: Karyotypio changes in callus cultures from haploid and diploid plants ofCrepis capillaris (L.) Wallr. -Chromosoma33: 273-283, 1971. Go to original source...
    7. Sheridan, W. F.: Plant regeneration and chromosome stability in tissue culture. - In: Ledoux, L. (ed.): Genetic Manipulation with Plant Material. Pp. 263-287. Plenum Press, London 1975. Go to original source...
    8. Singh, B. D.: Evidence of endoreduplication in callus cultures ofCrepis suffreniana Steud. - Indian. J. exp. Biol.12: 289-291, 1974.
    9. Torrey, J. G.: Morphogenesis in relation to chromosomal constitution in long term plant tissue cultures. - Plant Physiol.30: 265-275, 1967. Go to original source...

    Return to the content