Biologia plantarum 31:121-125, 1989 | DOI: 10.1007/BF02907242
Regeneration responses of callus from different expiants and changes in isozymes during morphogenesis in wheat
- 1 Plant Breeding Department, G. B. Pant University, Pantnagar, U. P., India
Immature embryo and root meristem expiants of wheat were cultured on modified medium of Murashige and Skoog and Gamborg's medium supplemented with 2,4-dichlorophenoxy acetic acid. Morphogeriic callus cultures were obtained from both the expiants. The frequency of shoot formation varied from 22% to 48% from callus obtained from embryos while only root formation could be induced from root meristem expiants. Cultures from young and old non-differentiating calli, and calli with shoot and/or root formation at different intervals were analysed for isozymes of esterase, peroxidase and acid phosphatase for studying the morphogenic capacity. With the development of shoot and/or root from callus, some conspicuous isozymes appeared which indicates the involvement of these isozymes in root/shoot development rather than in the induction of morphogenesis in callus. Basic isozyme pattern of each enzyme for the callus was retained in all the callus stages.
Received: October 6, 1987; Accepted: September 8, 1988; Published: March 1, 1989 Show citation
References
- Ahn, B. J.,Huang, F. M.,King, J. W. : Isoenzyme markers to differentiate embryogenic from non-embryogenic callus in bermudagrass tissue culture. -In vitro 21 : 11, 1985.
- Bergmann, J. W.,Mann, S. S. : Genetic control of isozymes in wheat-rye addition lines with rye or wheat cytoplasm. - In :Sears, E. R.,Sears, L. M. S. (ed.) : Proc. 4th Int. Wheat Genetics Symposium. Pp. 329-335. Missouri Publ. 1973.
- Chawla, H. S., Wenzel, G.:In vitro selection of barley and wheat for resistance againstHelminthos- porium sativum. - Theor. appl. Genet.74 : 841-845, 1987a.
Go to original source... - Chawla, H. S., Wenzel, G.: Regeneration potential of callus from wheat and barley. - Arch. Züchtungsforsch.17: 337-343, 1987b.
- Eapen, S., Rao, P. S.: Plant regeneration from immature inflorescence callus cultures of wheat, rye and triticale. - Euphytica34 : 153-159, 1985. Go to original source...
- Everett, N. P., Wach, M. J., Ashworth, D. J.: Biochemical markers of embryogenesis in tissue cultures of the maize inbred B 73. - Plant Sci.41 : 133-140, 1985. Go to original source...
- Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements of suspension cultures of soyabean root cells. - Exp. Cell Res.50 : 148-151, 1968. Go to original source...
- Khavkin, E. E., Sukhorvheskia, T. B.: Maintenance of isoenzyme spectra in callus and suspension cultures derived from internodes of maize (Zea mays L.). - Biochem. Physiol. Pflanzen174 : 431-437, 1979. Go to original source...
- Lörz, H., Brown, P., Gobel, E., Junker, B., Luhrs, R., Stolarz, A., Zimmy, J.:In vitro manipulation of cereal crops. - In :Semal, J. (ed.) : Somaclonal Variations and Crop Improvement. Pp. 117-186. Martinus Nijhoff Publishers, Dordrecht 1986. Go to original source...
- Marselak, L.: [Peroxidase isoenzyme patterns in tobacco callus cultures after the application of various growth regulators.] In Germ. - Acta Univ. Agr.29 : 15-23, 1981.
- Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. -Physiol. Plant.15: 473-497, 1962. Go to original source...
- Prasanna, P. S., Rao, K. V., Reddy, G. M.: Esterase isozyme studies in callus cultures. -Maize Genet. Newslett.57 : 52, 1983.
- Scandalios, K. W.: Genetic control of multiple molecular forms of enzymes in plants : A review. - Biochem. Genet.3 : 37-79, 1969. Go to original source...
- Scandalios, J. W.: Isozymes in development and differentiation. - Annu. Rev. Plant Physiol.25 : 225-258, 1974. Go to original source...
- Sears, R. G., Deckard, E. L.: Tissue culture variability in wheat: Callus induction and plant regeneration. -Crop Sci.22: 546-550, 1982. Go to original source...