Biologia plantarum 31:312, 1989 | DOI: 10.1007/BF02907293
Potato transformation byAgrobacterium rhizogenes Ri plasmid
- 1 Institute of Experimental Botany, Czechoslovak Academy of Sciences
- 2 Institute of Potato Research and Breeding, Havlíčkův Brod, Czechoslovakia
Potato root cultures transformed bypRiA4 T-DNA do not regenerate plants in vitro spontaneously, but regeneration can be induced by a sequence of callogenesis and regeneration media. Both Tl and Tr-DNA were found by Southern blotting. Regenerated transformants showed characteristic morphological deviations in the invitro cultures. Plants in the soil showed differences in the inflorescence and tuber development.
Received: August 5, 1988; Accepted: January 27, 1989; Published: July 1, 1989 Show citation
References
- An, G.,Watson, B. D.,Stachel, S.,Gordon, M. P.,Nester, E. W.: New cloning vehicles for transformation of higher plants. - EMBO J.4: 277-284, 1985.
Go to original source... - Cardarelli, M.,Spano, L.,Mariotti, D.,Mauro, M. L.,Van Sluyss, M. A.,Costanttno, P.: The role of auxin in hairy root induction. - Mol. gen. Genet.208: 457-463, 1987.
Go to original source... - Huffman, G. A.,White, F. F.,Gordon, M. P.,Nester, E. W.: Hairy root inducing plasmid: physical map and homology to tumor inducing plasmids. - J. Bacteriol.157: 269-276, 1984.
Go to original source... - Koplow, J.,Byrne, M. G.,Tempe, J.,Chilton, M. D.: Physical map of theAgrobacterium rhizogenes strain 8196 virulence plasmid. - Plasmid11: 17-27, 1984.
Go to original source... - Murashige, T.,Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. -Physiol. Plant.15: 473-497, 1962.
Go to original source... - Ondřej, M.,BÍSKOVÁ, R.: Plant regeneration fromAgrobacterium rhizogenes induced tumors.- In:Procházka, S.,Hradilík, J. (ed.): Regulation of Plant Integrity. [Acta Univ. Agr.] Sb. vys. ©koly zeměd. Brno, Řada A, 33. Pp. 475-477, Brno 1985.
- Ondřej, M.,Prottva, J.:In vitro culture of crown gall and hairy root tumors ofAtropa belladonna. Differentiation and alkaloid production. - Biol. Plant.29: 241-246, 1987.
Go to original source... - Ondřej, M.,Vlasák, J.: Experimental protocol of optimalization of leaf disc method ofAgrobacterium transformation for individual species of cultured plants. - Genet. Siecht. (Praha)25: 1-10, 1989.
- Ooms, G.,Karp, A.,Burrell, M. M.,Twell, D.,Roberts, J.: Genetic modification of potato development using Ri T-DNA. - Theor, appl. Genet.70: 440-446, 1985.
Go to original source... - Petit, A.,David, C.,Dahl, G. A.,Ellis, J. G.,Guyon, P.,Casse-Delbart, F.,Tempé, J.: Further extension of the opine concept: plasmids inAgrobacterium rhizogenes cooperate for opine degradation. - Mol. gen. Genet.190: 204-215, 1983.
Go to original source... - Rogers, S. G.,Horsch, R. B.,Fraley, R. T.: Gene transfer in plants: production of transformed plants using Ri plasmid vectors. - Methods Enzymol.118: 627-640, 1986.
Go to original source... - Webb, K. J.,Osifo, E. O.,Heshaw, G. G.: Potato tissue culture conditions. - Plant Sci. Lett.30:1-8, 1983.
Go to original source... - White, F. F.,Taylor, B. H.,Huffman, G. A.,Gordon, M. P.,Nester, E. W.: Molecular and genetic analysis of the transferred DNA region of the root-inducing plasmid ofAgrobacterium rhizogenes. - J. Bacteriol.164: 33-44, 1985.
Go to original source...



