biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 31:327-335, 1989 | DOI: 10.1007/BF02876346

The comparison between membrane and transorgan electric potentials inChenopodium rubrum: The methods

L. Adamec1
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6, Czechoslovakia

A new method of simultaneous measurement of membrane (Em) and transorgan (Etr)electric potential in intactChenopodium rubrum plants and changes in Em and Etr under various experimental conditions are described. Em was measured in mesophyll leaf cells, and Etr in the same plant as a potential difference between a first pair leaf, tip and roots. The two potentials differed distinctly, Em averaging-156mV and Etr -5mV. But the,-changes in Em and Etr had approximately the same magnitude and time-course after changing light and darkness or KCl concentration in solution flowing around the leaf or during the photoperiodic induetive cycle. In relation to the same electrode connection they had an opposite polarity. The nature of Etr and its relation to Em are discussed.
The measurement of Etr is much more stable and simpler than that of Em. The similarity of Em and Etr time-courses justifies the use of Etr measurement in electrophysiological studies as a replacement for Em measurements especially in intact plants.

Received: November 27, 1988; Accepted: January 26, 1989; Published: September 1, 1989  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Adamec, L. (1989). The comparison between membrane and transorgan electric potentials inChenopodium rubrum: The methods. Biologia plantarum, 31(5), 327-335. doi: 10.1007/BF02876346
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adamec, L., Krekule, J.: Changes in membrane potential inChenopodium rubrum during the course of photoperiodic flower induction. - Biol. Plant.31: 336-343, 1989a. Go to original source...
    2. Adamec, L., Krekule, J.: Changes in transorgan electric potential inChenopodium rubrum during the course of photoperiodic flower induction. - Biol. Plant.31: 344-353,1989b. Go to original source...
    3. Dunlop, J., Bowling, D. J. F.: The movement of ions to the xylem exudate of maize roots. III. The location of the electrical and electrochemical potential differences between the exudate and the medium. - J. exp. Bot.22: 453-464,1971. Go to original source...
    4. Jaffe, L. F., Nucoteixi, R.: Electrical controls of development. - Annu. Rev. Biophys. Bioeng.6:445-476,1977. Go to original source...
    5. Monta von, M.: Lumière et biopotentiels chez l'épinard(Spinacia oleracea L. cv. Nobel): phytochrome, communication interorganique et floraison. - PhD. Thesis, No. 2099. Univ. Genève 1984.
    6. Monta von, M., Horwttz, B. A., Greppin, H.: Far-red light-induced changes in intracellular potentials of spinach mesophyll cells. Interaction with red light. - Plant Physiol.73: 671-676,1983.
    7. Newman, I. A., Briggs, W. R.: Phytochrome-mediated electric potential changes in oat seedlings. - Plant Physiol.50: 687-693,1972. Go to original source...
    8. Novak, B., Bentrup, F. W.: An electrophysiological study of regeneration in Acetabulariamediter- ranea. -Planta108: 227-244, 1972. Go to original source...
    9. Novak, B., Greppin, H.: High-frequency oscillations and circadian rhythm of the membrane potential in spinach leaves. - Planta144: 235-240, 1979. Go to original source...
    10. Okamoto, H., Ichino, K., Katou, K.: Radial electrogenic activity in the stem of Vignasesquipedalis: involvement of spatially separate pumps. - Plant Cell Environ.1: 279-284, 1978. Go to original source...
    11. Okamoto, H., Katou, K., Mizuno, A., Kojima, H., Matsamura, Y., Ono, Y: Osmotic and hormonal control of electrogenic ion pumps and growth in pea hypocotyl segments by pressurized intra-organ perfusion. - In:Cram, W. J., Janáček, K., Rybová, R., Sigler, K. (ed.): Membrane Transport in Plants. Pp. 447-452. Academia, Praha 1984.
    12. Polevoi, V. V.: Rol Auksina v Sistemakh Regulyatsii u Rastenií. [The Role of Auxin in Regulatory Systems in Plants.] -Nauka, Leningrad 1986.
    13. Raven, J. A.: The possible role of membrane electrophoresis in the polar transport of IAA and other solutes in plant tissues. -New Phytol.78: 285-291,1979. Go to original source...
    14. Starrach, N., Freudling, G, Mayer, W. E., Gradmann, D.: Aged preparations are electrophysio- logically more representative of intact tissue than are freshly excised preparations. - Planta160: 88-90,1984 Go to original source...
    15. Ullmann, J., Seidlova, F., Krekule, J., Pavlova, L.:Chenopodium rubrum as a model plant for testing the flowering effects of PGRs. - Biol. Plant.27: 367-372, 1985. Go to original source...
    16. Zawadski, T., Trebacz, K.: Extra- and intracellular measurements of action potentials in the liverwort Conocephalumconicum. - Physiol. Plant.64: 477-481, 1985. Go to original source...

    Return to the content