Biologia plantarum 32:77-80, 1990 | DOI: 10.1007/BF02897348
Time course of electrolyte leakage from various samples killed by frost, liquid nitrogen or boiling
- 1 Department of Plant Physiology, Research Institute of Plant Production, Praha 6, Czechoslovakia
Samples of the same kind (rape leaves, wheat crowns, onion bulbs or spruce needles) killed by a slow freeze-thaw cycle or by immersion in liquid nitrogen showed a similar time course of leakage. Leakage was accelerated in treatments when the sample, immersed in water, was killed by heating for 5, 15 or 30 min in a boiling water bath. The longer the boiling, the sooner the maximum conductivity (Lkm) was obtained. The Lkm did not depend on the way of killing but in samples killed by freezing, it was achieved after a longer leakage time. The leakage rate decreased from onion to rape, wheat and spruce. The relative conductivity [%] allowed a better comparison of various treatments and of samples of different kinds of tissues than the specific conductivity [μS cm-1].
Received: February 23, 1989; Accepted: May 15, 1989; Published: January 1, 1990 Show citation
References
- Aronsson, A., Eliasson, L.: Frost hardiness in Scots pine (Pinus silvestris L.). I. Conditions for test on hardy plant tissues and for evaluation of injuries by conductivity measurements. - Stud. forest. suec.77: 1-30, 1970.
- Flint, H. L., Boyce, B. R., Beattie, D. J.: Index of injury - a useful expression of freezing injury to plant tissues as determined by the electrolytic method. - Can. J. Plant Sci.47: 229-230, 1967.
Go to original source... - Hallam, P. M., Tibbits, W. N.: Determination of frost hardiness ofEucalyptus using the electrical conductivity of diffusate in conjunction with a freezing chamber. - Can. J. Forest Res.18: 595-600, 1988. Go to original source...
- Levitt, J.: Responses of Plants to Environmental Stresses. Vol. 1 and 2. - Academic Press, Orlando - London 1980.
- Sullivan, C. Y.: Mechanisms of heat and drought resistance in grain sorghum and methods of measurement. - In:Rao, N. G. P., House, L. R. (ed.):Sorghum in Seventies. Pp. 247-262. Oxford and India Book House Publishing Co., New Delhi 1972.