biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 32:198, 1990 | DOI: 10.1007/BF02890873

Phaseolus coccineus L. Storage Proteins. Extraction and Characterization

R. Bernardi1, Maria C. Lupi1, M. Durante2
1 Department of Agricultural Plant Biology, Genetics Section, University of Pisa, Pisa
2 Institute of Agricultural and Forestal Chemistry, The University, Gallina, Reggio Calabria, Italy

Phaseolus coccineus storage globulins were extracted from mature cotyledons, purified and characterized. Three major proteins were separated. A component showing erythroagglutinating activity was thoroughly purified by thyroglobulin-Sepharose chromatography. The relative molecular masses of the three fractions are Mr = 330, 178, and 500 kDa as determined by polyacrylamide gel electrophoresis (PAGE). They correspond to the proteins found in other systems and classified as phytohaemagglutinin (PHA), vicilin and legumin, respectively. Electrophoretic analyses under denaturating conditions (SDS-PAGE) evidenced the major subunits for the three proteins. Isoelectrofocusing of the isolated proteins indicated a large heterogeneity for vicilin.

Received: August 1, 1989; Accepted: September 14, 1989; Published: May 1, 1990  Show citation

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Bernardi, R., Lupi, M.C., & Durante, M. (1990). Phaseolus coccineus L. Storage Proteins. Extraction and Characterization. Biologia plantarum, 32(3), 198. doi: 10.1007/BF02890873
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