biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 32:401, 1990 | DOI: 10.1007/BF02890884

Potato transformation by T-DNA Cytokinin synthesis gene

M. Ondrej3, Ivana MachÁĉková1, J. ĈatskÝ2, J. Eder1, M. Hrouda3, Jana Pospisilová2, Helena Synkova2
1 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha 6
2 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1
3 Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ĉeské Budějovice, Czechoslovakia

Potato plants were transformed by the A.tumefaciens vector which integrates into the plant genome two foreign genes only: pTi C58 T-DNA gene 4 (ipt) responsible for elevated synthesis of cytokinins and kanamycin resistance gene. Three teratoma clones studied showed approximately 3 times, 6 times and 9 times increased levels of zeatin riboside and zeatin in comparison with untransformed controls and slight increase of the IAA level. Shoots formed roots in agar cultures sporadically, if the increase of zeatin riboside and zeatin levels was not higher than 6 times the control level. The chlorophyll content and net photosynthetic rate decreased with increasing levels of zeatin and zeatin riboside.

Received: September 8, 1989; Accepted: January 11, 1990; Published: November 1, 1990  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ondrej, M., MachÁĉková, I., ĈatskÝ, J., Eder, J., Hrouda, M., Pospisilová, J., & Synkova, H. (1990). Potato transformation by T-DNA Cytokinin synthesis gene. Biologia plantarum, 32(6), 401. doi: 10.1007/BF02890884
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akiyoshi, D. E., Morris, R. O., Hinz, R., Mischke, B. S., Kosuge, T., Garfinkel, D. J., Gordon, M. P., Nester, E. W.: Cytokinin/auxin balance in crown gall tumors is regulated by specificloci in the T-DNA. - Proc. nat. Acad. Sci. USA80: 407-411, 1983. Go to original source...
    2. Arnon, D. I.: Copper'enzymes in chloroplasts. Polyphenoloxidases inBeta vulgaris.- Plant Physiol.24: 1-14, 1949. Go to original source...
    3. ĈatskÝ, J., TichÁ, I.: A closed system for measurement of photosynthesis, photorespiration and transpiration rates - Biol. Plant.17: 405-410, 1975.
    4. Eder, J., Rovenská, J., KutÁĈek, M., Ĉermák, V.: HPLC analysis of indole compounds inAgrobac- terium and transformed tobacco cells - In:KutÁĈek, M., Bandurski, R. S., Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants Pp. 389-390 Academia Praha; SPB Academic Publishing, The Hague 1988.
    5. Flores, S., Tobin, E. M.: Benzyladenine modulation of the expression of two genes for nuclear-encoded chloroplast proteins inLemna gibba: Apparent post-transcriptional regulation - Plant168: 349, 1986. Go to original source...
    6. Leemans, J., Deblaere, R., WnxMrrzER, L., de Greve, H., Hernalsteens, J. P., van Montagu,M., Schell, J.: Genetic identification of functions of TL-DNA transcripts in octopine crown galls - EMBO J.1: 147-152, 1982. Go to original source...
    7. Maniatis, T., FrItsch, E. F., Sambrook, J.: Molecular Qoning: A Laboratory Manual - Cold Spring Harbor Lab, Cold Spring Harbor, New York 1982.
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures -Physiol. Plant.15: 473-497, 1962. Go to original source...
    9. Ondrej, M.,Bavrina, T. V.,Dudko, N.,Hrouda, M.,Krekule, J.,Lozhnikova, V. N.,MachÁĉková, I.,SeidlovÁ, F.,VlasÁk, J.: Transgenic tobacco plants with T-DNA phytohormone synthesis genes - Biol. Plant., (in press) 1990. Go to original source...
    10. Ondrej, M., Hrouda, M., Karavajko, N. N., Matouŝek, J., Mikuloviĉ, T. P., PavingerovÁ, D., VlasÁk, J.: Transformation byAgwbacterium vectors and the study of functions of plant hormones. - In: Krekule, J., Seidlova, F. (ed.): Signals in Plant Development Pp. 73-89 SPB Academic Publishing The Hague 1989.
    11. Ooms, G., Lenton, J. R.: T-DNA genes to study plant development: precocious tuberization and enhanced cytokinins inA. tumefaciens transformed potato - Plant mol.Biol.5: 205-212, 1985. Go to original source...
    12. Partier, B., Lerbs, S., Lehmann, J., Wollgiehn, R.: Cytokinin-controlled ribulose l, 5-bisphosphate carboxylase gene expression in pumpkin cotyledons - Biol. Plant27: 131-138, 1985. Go to original source...
    13. PospíŝilovÁ, J., TichÁ, I., ĉatský, J., SolÁrovÁ, J., Ondrej, M.: Photosynthesis in regenerants of tobacco transformed by plasmids ofAgrobacterium 3. Transplanted plants - Photosynthetica23: 130-135, 1989.
    14. Roger, S. O., Bendich, A. J.: Extraction of DNA from milligram amounts of fresh, herbarious and mummified plant tissues - Plant mol. Biol.5: 69-73, 1985. Go to original source...
    15. Rogers, S. G., Horsch, R. B., Fraley, R. T.: Gene transfer in plants. Production of transformed plants using Ti plasmid vectors - Methods Enzymol118: 627-641, 1986. Go to original source...
    16. Strnad, M., Vanek, T., BinárovÁ, P., Kamínek, M., Hanuŝ, J.: Enzyme immunoassays for cytokinins in plants and their use for immunodetection of cytokinins in alfalfa cell cultures - In:KutÁĉek, M., Elliott, L. O., MachÁĉková, I.: Molecular Aspects of Hormone Regulation of Plant Development - Pp. 41-54 SPB Academic Publishing, The Hague 1989.
    17. van Onckelen, H., Rüdelsheim, P., Beinsberger, S., Inze, D., van Montagu, M., de Greef, J.: Mutual interaction of plant specific and T-DNA controlled IAA metabolism in transformed tobacco tissue. - In:KutÁĈek, M., Bandurski, R. S., Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants Pp. 379-387 Academia, Praha; SPB Academic Publishing, The Hague 1988.

    Return to the content