biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 33:40-48, 1991 | DOI: 10.1007/BF02873786

Transgenic Tobacco plants with T-DNA Phytohormone synthesis genes

M. OndŘej1, Tamara V. Bavrina2, Natalja Dudko2, M. Hrouda3, J. Krekule3, Veronika N. Lozhnikova2, Ivana Machácková3, Frideta Seidlová3, J. Vlasák1
1 Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, České Budějovice, Czechoslovakia
2 Institute of Plant Physiology of A. K. Timiryazev, Academy of Sciences of USSR, Moscow, USSR
3 Research Institute for Crop Production, Praha 6, Czechoslovakia

Agrobacterium tumefaciens binary vectors carrying kanamycin resistance gene and either C58 T-DNA gene 4 for cytokinin synthesis or genes 1 and 2 for auxin synthesis were constructed and used for transformation of a short-day tobacco Maryland Mammoth. Kanamycin resistant plants were regenerated from a small fraction of transformed tissue and the presence of T-DNA in their genome was verified by Southern blotting. The level of endogenous cytokinin in plants transgenic for gene 4 and the level of endogenous IAA in those transgenic for genes 1 and 2 increased by more than 100 %. A number of morphological characteristics distinguish them from untransformed controls.

Received: December 7, 1989; Accepted: April 5, 1990; Published: January 1, 1991  Show citation

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OndŘej, M., Bavrina, T.V., Dudko, N., Hrouda, M., Krekule, J., Lozhnikova, V.N., ... Vlasák, J. (1991). Transgenic Tobacco plants with T-DNA Phytohormone synthesis genes. Biologia plantarum, 33(1), 40-48. doi: 10.1007/BF02873786
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