biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 33:175-180, 1991 | DOI: 10.1007/BF02897878

Regeneration potentiality and isozymic variations during morphogenesis of barley callus

H. S. Chawla1
1 Plant Breeding Dept., G. B. Pant University, Pantnagar U.P., India

Morphogenic callus cultures were obtained from 7-10 days old immature embryo explants on Murashige and Skoog and Gamborg's medium supplemented with 2,4-D. In the initial stages of culture the frequency of shoot formation varied from 28% to 65%. After 5 to 6 months of subculturing, the frequency of shoot formation was reduced to 14%. In the initial stages of culture, growth hormones do not seem to be very important for regeneration. Cultures from young and old non-differentiating calli, and calli with shoot and/or root formation at different intervals were analysed for isozymes of esterase, peroxidase and acid phosphatase for studying the morphogenic capacity. With the development of shoot/root, changes in isozymes takes place but no specific isozyme(s) could be related to the process of induction of morphogenesis.

Keywords: Hordeum vulgare; plant regeneration; esterase; peroxidase; acid; phosphatase

Received: October 7, 1988; Accepted: January 29, 1990; Published: May 1, 1991  Show citation

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Chawla, H.S. (1991). Regeneration potentiality and isozymic variations during morphogenesis of barley callus. Biologia plantarum, 33(3), 175-180. doi: 10.1007/BF02897878
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